Figure 4. Dendogram of native bacterial strain similarity based on analysing 16S ribosomal gene partial sequences.
The dendogram was constructed from the 1,400 to 1,500 bp 16S rDNA gene sequences by the Neighbour-joining method using the P distance with MEGA 3.0 software (Kumar et al. 2004). External group, B. megaterium. The dendogram shows five clusters. The first cluster corresponds to bacteria from the Pseudomonas genera (Anzai et al. 2000). The second cluster, formed by bacterial species from the Enterobacteriaceae family, belonged to IBUN N2004. IBUN N3510 was in the third cluster, similar to S. maltophilia. The fourth cluster contained IBUN 2G-57, closely related to Burkholderia cepacia. The fifth cluster was observed to be closely related to IBUN B69 with species from the Rhizobium genera.