Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 3, Issue of August 15, 2007
 


Figure 3. Colony PCR amplification of the phaC1 and phaC2 genes in reference bacteria and native bacteria from the old collection.

(a) Amplifying the phaC1 gene using E1-D and E1-R primers.

(b) Amplifying the phaC2 gene using E2-D and E2-R primers.

Lane 1, molecular weight marker (l ADN HindIII); 2, Pseudomonas fluorescens IBUN 91; 3, Pseudomonas sp. IBUN C14; 4, Pseudomonas sp. IBUN C16; 5, Pseudomonas sp. IBUN S0804; 6, Burkholderia sp. IBUN S1602; 7, Pseudomonas sp. IBUN S1804; 8, Pseudomonas sp. IBUN 1G-3*; 9, E. coli XL-1 Blue (negative control); 10, reagent control.


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