Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 3, Issue of August 15, 2007
 


Figure 2. PCR amplification of phaC gene for control strains.

(a) Colony PCR amplification of a phaC gene fragment using G-D and G-1R primers. Lane 1, molecular weight marker (λ ADN HindIII); 2, R. eutropha ATCC 17699; 3, A. hydrophyla IBUN 89; 4, A. brasilense ATCC 29710; 5, A. baumanni IBUN 90; 6, P. aeruginosa IBUN 1040; 7, P.aeruginosa ATCC 27853; 8, P. fluorescens IBUN 91; 9, P. putida IBUN 92;10, A. chroococcum IBUN 88; 11, A. amazonense IBUN 87; 12, E. coli XL-1 blue (negative control); 13, reagent control.

(b)Confirmation by semi-nested PCR using G-D and G-2R primers. Lane 1, molecular weight marker (λ ADN HindIII); 2, P. aeruginosa ATCC 27853 (phaC+); 3, P. putida IBUN 92(phaC+); 4, A. amazonense IBUN 87; 5, A. chroococcum IBUN 88; 6, E. coli XL-1 blue (negative control); 7, reagent control.


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