Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 2, Issue of April 15, 2007

Figure 1. Method for functional complementation under yeast promoter control. Amplification of the Upstream and Downstream Regulatory Regions (URR and DRR) is followed by their ligation. This ligation product is amplified using the yORF-F and yORF-R primers and inserted in pRS416 by BamHI ligation. The human Open reading frame (BC002639) can subsequently be cloned in the Bg/II site between the regulatory regions. Thus, extraneous sequences are limited to 6 bp in the downstream region and 2 codons at the 5' of the ORF.

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