Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 2, Issue of April 15, 2007
 

Table 2. Purification of tannase isolated from Paecilomyces variotii.


Purification step

Total activity
(U)

Total protein
(mg)

Specific activity
(U.mg-1)

Purification
(fold)

Yield
(%)

Crude enzyme

90

307,60

0,29

1

100

Ammonium sulphate

30,77

54,74

0,56

1,93

34

DEAE-Sepharose

2,7

0,48

5,6

10

3


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