Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 2, Issue of April 15, 2007
 

Table 1. Effect of Tween 80, Tween 20 and Triton X-100, chelator and inhibitors on crude tannase activity.


Additives

Concentration
(%(v/v))

Relative activity
(%)

Control

-

100 0.64

Tween 80

0.25

85 1.83


0.5

77 1.53


1.0

70 1.84

Tween 20

0.25

81 2.12


0.5

75 2.44


1.0

66 0.91

Triton X-100

0.25

96 0.31


0.5

93 0.61


1.0

87 0.62

Control

-

100 2.67

EDTA

1 mM

62 3.59

Sodium bisulphite

1 mM

75 4.40

Iodoacetamide

1 mM

96 1.26

2-mercaptoethanol

1 mM

59 3.14

4-aminobenzoic acid

1 mM

75 3.45

Sodium azide

1 mM

64 3.14

N- bromosuccinimide

1 mM

71 5.60

Cysteine

1 mM

52 3.14


Supported by UNESCO / MIRCEN network