Figure 2. Kinetics of the production of endochitinases by T. aureoviride wt (a) and mutant 7-121 (b). Native PAGE was carried out in 15% polyacrylamide gels at pH 8.8. 100 µL of supernatants of chitin-grown cultures were loaded into each well. Lanes 1, 2, 3, 4, and 5 correspond to culture supernatants at 5, 7, 9, 11 and 13 days of culture, respectively.